Polycyclic aromatic hydrocarbon (PAHs) are highly hydrophobic and are one of the most common and most difficult organic pollutants in the environment. China is PAHs emissions, it is estimated that the annual emissions of over 100,000 tons, and increased year by year. PAHs in the environment mainly come from the oil spill, automobile exhaust, pesticide waste, fossil fuel and biomass incomplete combustion, soil PAHs mainly from air pollution, sewage irrigation, sludge farmland and so on. The low toxicity or non-toxicity of polycyclic aromatic hydrocarbon (PAHs) in the environment through microbial degradation is one of the hotspots in environmental remediation.
Recently, the Chinese Academy of Sciences, Shenyang Institute of Applied Ecology published in Soil Biology & Biochemistry on an article, reported polycyclic aromatic hydrocarbon microbial degradation mechanism. The long-term rhizosphere effect of ryegrass was studied by PCR-DGGE, cloning and quantitative PCR, and the effects of long-term rhizosphere effect on PAHs degradation, functional bacterial community structure, The number of degrading bacteria and the expression of RHDα gene.
The results showed that the soils were rich in Gram-positive and negative PAHs, RHDα genes contained nidA3, pdoA, nahAc and phnAc, and the gram-positive bacteria of ryegrass rhizosphere PAHs were mainly Gram-positive Planting in 0 to 10 days to increase the diversity of functional bacteria, the bacteria species have a selective role in promoting. The effects of ryegrass and mycobacteria can promote the degradation of PAHs in farmland by changing the microbial community structure, increasing the diversity of bacteria and stimulating the expression of double oxygenase gene at different times. This study provides a basis for understanding the degradation of PAHs by rhizosphere effect of ryegrass, and provides a theoretical basis for the further development of remediation theory and technology of PAHs contaminated soil from the molecular level to understand the plant-microbial relationship in contaminated soil remediation process.
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